13-HODE inhibits the intracellular calcium increase of activated human polymorphonuclear cells

M.J. Van De Velde; F. Engels; P.A.J. Henricks; F.P. Nijkamp

13-HODE inhibits the intracellular calcium increase of activated human polymorphonuclear cells

M.J. Van De Velde, F. Engels, P.A.J. Henricks, F.P. Nijkamp

Pharmacology

External unknown

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We studied the effect of the linoleic acid metabolite 13-hydroxyoctadecadienoic acid (13-HODE) on the increase of the intracellular calcium concentration, [Ca2+](i), induced by platelet-activating factor (PAF), leukotriene By (LTB4), and formyl-Met-Leu-Phe (fMLP) in human polymorphonuclear leukocytes (PMNs). 13-HODE by itself did not change the [Ca2+](i) of PMNs. However, when PMNs were preincubated with 13-HODE and subsequently stimulated with PAF, LTB4, or fMLP, an inhibition of the increase of the [Ca2+](i) was observed. The PAF-induced calcium response was concentration-dependently inhibited between 0.1 and 5 μM 13-HODE. These results suggest that 13-HODE can affect the increase of the [Ca2+](i) and hence can modulate activation of PMNs.

Original language	English
Pages (from-to)	200-202
Number of pages	3
Journal	Journal of Leukocyte Biology
Volume	56
Issue number	2
Publication status	Published - 26 Jan 1994

Keywords

Calcium
Linoleic acid
Platelet-activating factor
Signal transduction
13 hydroxyoctadecadienoic acid
calcium
fatty acid derivative
formylmethionylleucylphenylalanine
leukotriene B4
thrombocyte activating factor
unclassified drug
article
cell stimulation
controlled study
human
human cell
neutrophil
priority journal
signal transduction

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title = "13-HODE inhibits the intracellular calcium increase of activated human polymorphonuclear cells",

abstract = "We studied the effect of the linoleic acid metabolite 13-hydroxyoctadecadienoic acid (13-HODE) on the increase of the intracellular calcium concentration, [Ca2+](i), induced by platelet-activating factor (PAF), leukotriene By (LTB4), and formyl-Met-Leu-Phe (fMLP) in human polymorphonuclear leukocytes (PMNs). 13-HODE by itself did not change the [Ca2+](i) of PMNs. However, when PMNs were preincubated with 13-HODE and subsequently stimulated with PAF, LTB4, or fMLP, an inhibition of the increase of the [Ca2+](i) was observed. The PAF-induced calcium response was concentration-dependently inhibited between 0.1 and 5 μM 13-HODE. These results suggest that 13-HODE can affect the increase of the [Ca2+](i) and hence can modulate activation of PMNs.",

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author = "{Van De Velde}, M.J. and F. Engels and P.A.J. Henricks and F.P. Nijkamp",

year = "1994",

month = jan,

day = "26",

language = "English",

volume = "56",

pages = "200--202",

journal = "Journal of Leukocyte Biology",

issn = "0741-5400",

publisher = "FASEB",

number = "2",

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TY - JOUR

T1 - 13-HODE inhibits the intracellular calcium increase of activated human polymorphonuclear cells

AU - Van De Velde, M.J.

AU - Engels, F.

AU - Henricks, P.A.J.

AU - Nijkamp, F.P.

PY - 1994/1/26

Y1 - 1994/1/26

N2 - We studied the effect of the linoleic acid metabolite 13-hydroxyoctadecadienoic acid (13-HODE) on the increase of the intracellular calcium concentration, [Ca2+](i), induced by platelet-activating factor (PAF), leukotriene By (LTB4), and formyl-Met-Leu-Phe (fMLP) in human polymorphonuclear leukocytes (PMNs). 13-HODE by itself did not change the [Ca2+](i) of PMNs. However, when PMNs were preincubated with 13-HODE and subsequently stimulated with PAF, LTB4, or fMLP, an inhibition of the increase of the [Ca2+](i) was observed. The PAF-induced calcium response was concentration-dependently inhibited between 0.1 and 5 μM 13-HODE. These results suggest that 13-HODE can affect the increase of the [Ca2+](i) and hence can modulate activation of PMNs.

AB - We studied the effect of the linoleic acid metabolite 13-hydroxyoctadecadienoic acid (13-HODE) on the increase of the intracellular calcium concentration, [Ca2+](i), induced by platelet-activating factor (PAF), leukotriene By (LTB4), and formyl-Met-Leu-Phe (fMLP) in human polymorphonuclear leukocytes (PMNs). 13-HODE by itself did not change the [Ca2+](i) of PMNs. However, when PMNs were preincubated with 13-HODE and subsequently stimulated with PAF, LTB4, or fMLP, an inhibition of the increase of the [Ca2+](i) was observed. The PAF-induced calcium response was concentration-dependently inhibited between 0.1 and 5 μM 13-HODE. These results suggest that 13-HODE can affect the increase of the [Ca2+](i) and hence can modulate activation of PMNs.

KW - Calcium

KW - Linoleic acid

KW - Platelet-activating factor

KW - Signal transduction

KW - 13 hydroxyoctadecadienoic acid

KW - calcium

KW - fatty acid derivative

KW - formylmethionylleucylphenylalanine

KW - leukotriene B4

KW - thrombocyte activating factor

KW - unclassified drug

KW - article

KW - cell stimulation

KW - controlled study

KW - human

KW - human cell

KW - neutrophil

KW - priority journal

KW - signal transduction

M3 - Article

SN - 0741-5400

VL - 56

SP - 200

EP - 202

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

IS - 2

ER -

13-HODE inhibits the intracellular calcium increase of activated human polymorphonuclear cells

Abstract

Keywords

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