α-D-Glcp-(1⇆1)-β-D-Galp-containing oligosaccharides, novel products from lactose by the action of β-galactosidase

Carel T.M. Fransen; Katrien M.J. Van Laere; Arjan A.C. Van Wijk; Lars P. Brüll; Mark Dignum; Jane E. Thomas-Oates; Johan Haverkamp; Henk A. Schols; Alphons G.J. Voragen; Johannis P. Kamerling; Johannes F.G. Vliegenthart

doi:10.1016/S0008-6215(98)00285-7

α-D-Glcp-(1⇆1)-β-D-Galp-containing oligosaccharides, novel products from lactose by the action of β-galactosidase

Carel T.M. Fransen
, Katrien M.J. Van Laere
, Arjan A.C. Van Wijk
, Lars P. Brüll
, Mark Dignum
, Jane E. Thomas-Oates
, Johan Haverkamp
, Henk A. Schols
, Alphons G.J. Voragen
, Johannis P. Kamerling
, Johannes F.G. Vliegenthart

Sub NMR Spectroscopy

Wageningen University & Research
Bijvoet Center

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A mixture of oligosaccharides produced by β-galactosidase using lactose as a substrate was fractionated according to degree of polymerization using gel filtration, followed by high-pH anion-exchange chromatography. The fractions obtained were analyzed using monosaccharide analysis, methylation analysis, mass spectrometry, and NMR spectroscopy. Twelve novel non-reducing oligosaccharides were characterized, namely, [β-D-Galp-(1→4)](n)-α-D-Glcp-(1⇆1)-β-D-Galp[-(4←1)-β-D-Galp](m), with n, m=(1, 2, 3, or 4) and β-D-Galp-(1→2)-α-D-Glcp-(1⇆1)-β-D-Galp. Copyright (C) 1998 Elsevier Science Ltd.

Original language	English
Pages (from-to)	101-114
Number of pages	14
Journal	Carbohydrate Research
Volume	314
Issue number	1-2
DOIs	https://doi.org/10.1016/S0008-6215(98)00285-7
Publication status	Published - 21 Dec 1998

Keywords

β-Galactosidase
Functional food
Non-reducing galacto-oligosaccharides
Oligogalactosylated glucoses
Primary structure analysis
Transgalactosylation
beta galactosidase
lactose
oligosaccharide
anion exchange chromatography
article
carbohydrate analysis
carbohydrate synthesis
chemical modification
gel filtration chromatography
mass spectrometry
methylation
nuclear magnetic resonance spectroscopy
polymerization
priority journal
reaction analysis

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10.1016/S0008-6215(98)00285-7

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Fransen, C. T. M., Van Laere, K. M. J., Van Wijk, A. A. C., Brüll, L. P., Dignum, M., Thomas-Oates, J. E., Haverkamp, J., Schols, H. A., Voragen, A. G. J., Kamerling, J. P., & Vliegenthart, J. F. G. (1998). α-D-Glcp-(1⇆1)-β-D-Galp-containing oligosaccharides, novel products from lactose by the action of β-galactosidase. Carbohydrate Research, 314(1-2), 101-114. https://doi.org/10.1016/S0008-6215(98)00285-7

@article{4c67d7452884403bbcd50c3db2d39a94,

title = "α-D-Glcp-(1⇆1)-β-D-Galp-containing oligosaccharides, novel products from lactose by the action of β-galactosidase",

abstract = "A mixture of oligosaccharides produced by β-galactosidase using lactose as a substrate was fractionated according to degree of polymerization using gel filtration, followed by high-pH anion-exchange chromatography. The fractions obtained were analyzed using monosaccharide analysis, methylation analysis, mass spectrometry, and NMR spectroscopy. Twelve novel non-reducing oligosaccharides were characterized, namely, [β-D-Galp-(1→4)](n)-α-D-Glcp-(1⇆1)-β-D-Galp[-(4←1)-β-D-Galp](m), with n, m=(1, 2, 3, or 4) and β-D-Galp-(1→2)-α-D-Glcp-(1⇆1)-β-D-Galp. Copyright (C) 1998 Elsevier Science Ltd.",

keywords = "β-Galactosidase, Functional food, Non-reducing galacto-oligosaccharides, Oligogalactosylated glucoses, Primary structure analysis, Transgalactosylation, beta galactosidase, lactose, oligosaccharide, anion exchange chromatography, article, carbohydrate analysis, carbohydrate synthesis, chemical modification, gel filtration chromatography, mass spectrometry, methylation, nuclear magnetic resonance spectroscopy, polymerization, priority journal, reaction analysis",

author = "Fransen, \{Carel T.M.\} and \{Van Laere\}, \{Katrien M.J.\} and \{Van Wijk\}, \{Arjan A.C.\} and Br{\"u}ll, \{Lars P.\} and Mark Dignum and Thomas-Oates, \{Jane E.\} and Johan Haverkamp and Schols, \{Henk A.\} and Voragen, \{Alphons G.J.\} and Kamerling, \{Johannis P.\} and Vliegenthart, \{Johannes F.G.\}",

year = "1998",

month = dec,

day = "21",

doi = "10.1016/S0008-6215(98)00285-7",

language = "English",

volume = "314",

pages = "101--114",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Elsevier Ltd",

number = "1-2",

}

Fransen, CTM, Van Laere, KMJ, Van Wijk, AAC, Brüll, LP, Dignum, M, Thomas-Oates, JE, Haverkamp, J, Schols, HA, Voragen, AGJ, Kamerling, JP & Vliegenthart, JFG 1998, 'α-D-Glcp-(1⇆1)-β-D-Galp-containing oligosaccharides, novel products from lactose by the action of β-galactosidase', Carbohydrate Research, vol. 314, no. 1-2, pp. 101-114. https://doi.org/10.1016/S0008-6215(98)00285-7

TY - JOUR

T1 - α-D-Glcp-(1⇆1)-β-D-Galp-containing oligosaccharides, novel products from lactose by the action of β-galactosidase

AU - Fransen, Carel T.M.

AU - Van Laere, Katrien M.J.

AU - Van Wijk, Arjan A.C.

AU - Brüll, Lars P.

AU - Dignum, Mark

AU - Thomas-Oates, Jane E.

AU - Haverkamp, Johan

AU - Schols, Henk A.

AU - Voragen, Alphons G.J.

AU - Kamerling, Johannis P.

AU - Vliegenthart, Johannes F.G.

PY - 1998/12/21

Y1 - 1998/12/21

N2 - A mixture of oligosaccharides produced by β-galactosidase using lactose as a substrate was fractionated according to degree of polymerization using gel filtration, followed by high-pH anion-exchange chromatography. The fractions obtained were analyzed using monosaccharide analysis, methylation analysis, mass spectrometry, and NMR spectroscopy. Twelve novel non-reducing oligosaccharides were characterized, namely, [β-D-Galp-(1→4)](n)-α-D-Glcp-(1⇆1)-β-D-Galp[-(4←1)-β-D-Galp](m), with n, m=(1, 2, 3, or 4) and β-D-Galp-(1→2)-α-D-Glcp-(1⇆1)-β-D-Galp. Copyright (C) 1998 Elsevier Science Ltd.

AB - A mixture of oligosaccharides produced by β-galactosidase using lactose as a substrate was fractionated according to degree of polymerization using gel filtration, followed by high-pH anion-exchange chromatography. The fractions obtained were analyzed using monosaccharide analysis, methylation analysis, mass spectrometry, and NMR spectroscopy. Twelve novel non-reducing oligosaccharides were characterized, namely, [β-D-Galp-(1→4)](n)-α-D-Glcp-(1⇆1)-β-D-Galp[-(4←1)-β-D-Galp](m), with n, m=(1, 2, 3, or 4) and β-D-Galp-(1→2)-α-D-Glcp-(1⇆1)-β-D-Galp. Copyright (C) 1998 Elsevier Science Ltd.

KW - β-Galactosidase

KW - Functional food

KW - Non-reducing galacto-oligosaccharides

KW - Oligogalactosylated glucoses

KW - Primary structure analysis

KW - Transgalactosylation

KW - beta galactosidase

KW - lactose

KW - oligosaccharide

KW - anion exchange chromatography

KW - article

KW - carbohydrate analysis

KW - carbohydrate synthesis

KW - chemical modification

KW - gel filtration chromatography

KW - mass spectrometry

KW - methylation

KW - nuclear magnetic resonance spectroscopy

KW - polymerization

KW - priority journal

KW - reaction analysis

U2 - 10.1016/S0008-6215(98)00285-7

DO - 10.1016/S0008-6215(98)00285-7

M3 - Article

SN - 0008-6215

VL - 314

SP - 101

EP - 114

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 1-2

ER -

α-D-Glcp-(1⇆1)-β-D-Galp-containing oligosaccharides, novel products from lactose by the action of β-galactosidase

Abstract

Keywords

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